Target protein identification in live cells and organisms with a non-diffusive proximity tagging system.

ABSTRACT: Identifying target proteins for bioactive molecules is essential for understanding their mechanisms, developing improved derivatives, and minimizing off-target effects. Despite advances in target identification (target-ID) technologies, significant challenges remain, impeding drug development. Most target-ID methods use cell lysates, but maintaining an intact cellular context is vital for capturing specific drug-protein interactions, such as those with transient protein complexes and membrane-associated proteins. To address these limitations, we developed POST-IT (Pup-On-target for Small molecule Target Identification Technology), a non-diffusive proximity tagging system for live cells, orthogonal to the eukaryotic system. POST-IT utilizes an engineered fusion of proteasomal accessory fact

SUBMITTER: Sun Y 

PROVIDER: S-EPMC11677243 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature