<i>Triglochin maritima</i> Extracts Exert Anti-Melanogenic Properties via the CREB/MAPK Pathway in B16F10 Cells.

ABSTRACT: Triglochin maritima, a salt-tolerant plant, has demonstrated antioxidant effects, the ability to prevent prostate enlargement, antifungal properties, and skin moisturizing benefits. This study aimed to explore the anti-melanogenic potential of the 70% ethanol extract of T. maritima (TME) along with its ethyl acetate (TME-EA) and water (TME-A) fractions. TME (10-200 µg/mL), TME-EA (1-15 µg/mL), and TME-A (100-1000 µg/mL) were prepared and applied to B16F10 cells with or without α-MSH for 72 h. MTT assays were used to assess cytotoxicity, and anti-melanogenesis activity was determined by measuring melanin content, conducting a tyrosinase activity assay, and evaluating the expression of melanogenesis-related genes and proteins via RT-PCR and Western blotting. HPLC-PDA was used t

SUBMITTER: Lee WH 

PROVIDER: S-EPMC11677928 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature