Discovery and Characterization of an Atypical Crustin Antimicrobial Peptide from Pollicipes pollicipes.

ABSTRACT: Crustins are a family of antimicrobial peptides (AMPs) that play a pivotal role in the innate immune system of crustaceans. The discovery of novel AMPs from natural sources is crucial for expanding our current database of these peptides. Here, we identified and characterized a novel member of the crustin family, named PpCrus-SWD1, derived from Pollicipes pollicipes. PpCrus-SWD1 consists of 138 amino acids and contains eight cysteine residues that form a conserved 'four-disulfide core' structure. Our recombinant PpCrus-SWD1 (rPpCrus-SWD1) exhibited potent inhibitory activity against three Gram-positive bacteria (Staphylococcus aureus, Bacillus sp. T2, and Streptococcus agalactiae) and six Gram-negative bacteria (Aeromonas hydrophila

SUBMITTER: Zhang W 

PROVIDER: S-EPMC11678330 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature