Enhanced Specificity in Colorimetric LAMP Assay for <i>Sarocladium kiliense</i> Detection Using a Combination of Two Additives.

ABSTRACT: The genus Sarocladium comprises fungal species closely related to Acremonium, with S. kiliense and S. strictum being medically important. These species can cause infections in both immunocompetent and immunocompromised individuals. The current detection methods are limited, prompting the need for rapid and specific diagnostic tools. We developed a colorimetric loop-mediated isothermal amplification (LAMP) assay targeting S. kiliense (SK-LAMP). The initial prototype assay faced challenges with cross-reactivities with closely related species. To address this, we incorporated two additives, pullulan and tetramethylammonium chloride (TMAC), which are known to reduce non-specific signals in amplification assays. Our study found that a combination of 1% (v</

SUBMITTER: Wong YN 

PROVIDER: S-EPMC11678401 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature