Nanopore Data-Driven Chromosome-Level Assembly of Flax Genome.

ABSTRACT: Flax is an important crop grown for seed and fiber. Flax chromosome number is 2n = 30, and its genome size is about 450-480 Mb. To date, the genomes of several flax varieties have been sequenced and assembled. However, the obtained assemblies are still far from the telomere-to-telomere (T2T) level. We sequenced the genome of flax variety K-3018 on the Oxford Nanopore Technologies (ONT) platform and obtained 57.7 Gb of R10 simplex reads with an N50 = 18.4 kb (~120× genome coverage). ONT reads longer than 50 kb were kept as ultra-long ones (~10× genome coverage), and the rest of the ONT reads were corrected using the HERRO R10 model (quality > Q10, length > 10 kb, ~60× genome coverage remained). The genome was assembled using Hifiasm and Verkko. The Hifiasm-generated assembly was 489.1 Mb in

SUBMITTER: Arkhipov AA 

PROVIDER: S-EPMC11679535 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature