Real-Time Observation of Clickable Cyanotoxin Synthesis in Bloom-Forming Cyanobacteria <i>Microcystis aeruginosa</i> and <i>Planktothrix agardhii</i>.

ABSTRACT: Recently, the use of click chemistry for localization of chemically modified cyanopeptides has been introduced, i.e., taking advantage of promiscuous adenylation (A) domains in non-ribosomal peptide synthesis (NRPS), allowing for the incorporation of clickable non-natural amino acids (non-AAs) into their peptide products. In this study, time-lapse experiments have been performed using pulsed feeding of three different non-AAs in order to observe the synthesis or decline of azide- or alkyne-modified microcystins (MCs) or anabaenopeptins (APs). The cyanobacteria Microcystis aeruginosa and Planktothrix agardhii were grown under maximum growth rate conditions (r = 0.35-0.6 and 0.2-0.4 (day^-1), respectively) in the presence of non-AAs for 12-168 h. The decline of the az

SUBMITTER: Kurmayer R 

PROVIDER: S-EPMC11679549 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature