Nanopore Data-Driven T2T Genome Assemblies of Colletotrichum lini Strains.

ABSTRACT: Colletotrichum lini is a pathogenic fungus that infects flax and causes significant yield losses. In this study, we assembled the genomes of four highly virulent C. lini strains using the Oxford Nanopore Technologies (ONT, R10.4.1 flow cells) and Illumina platforms. The performance of two tools developed for telomere-to-telomere (T2T) genome assembly was compared: Verkko and Hifiasm. Prior to the assembly, ONT reads were corrected using the HERRO algorithm. Verkko generated genome assemblies of high completeness but low contiguity, while Hifiasm allowed the generation of T2T assemblies. Despite significantly different genome coverage with ONT data (25-100×), four assemblies of equal contiguity were obtained: 53.6-54.7 Mb, ten core chromosomes, and two or three accessory chrom

SUBMITTER: Sigova EA 

PROVIDER: S-EPMC11679667 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature