Sperm Functional Status: A Multiparametric Assessment of the Fertilizing Potential of Bovine Sperm.

ABSTRACT: Sperm viability is routinely assessed for the quality control of cryopreserved bovine sperm batches but is not usually conclusive regarding their fertilizing potential. In this study, we investigated the fertility predictive value of bull sperm viability in combination with DNA integrity or the functional status of viable sperm. In addition to sperm viability, we flow cytometrically assessed the percentage of sperm with high DNA fragmentation index (%DFI) and the fraction of viable sperm with low intracellular Ca²⁺ content and functional mitochondria using the Sperm Chromatin Structure Assay and a five-color staining panel in 791 and 733 cryopreserved batches with non-return rate (NRR) records after ≥100 first services, respectively. Using linear mixed-effects models and conditi

SUBMITTER: Odinius TS 

PROVIDER: S-EPMC11680172 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature