Development of a Large-Volume Concentration Method to Recover Infectious Avian Influenza Virus from the Aquatic Environment.

ABSTRACT: Since late 2021, outbreaks of highly pathogenic avian influenza virus have caused a record number of mortalities in wild birds, domestic poultry, and mammals in North America. Wetlands are plausible environmental reservoirs of avian influenza virus; however, the transmission and persistence of the virus in the aquatic environment are poorly understood. To explore environmental contamination with the avian influenza virus, a large-volume concentration method for detecting infectious avian influenza virus in waterbodies was developed. A variety of filtering, elution, and concentration methods were explored, in addition to testing filtering speeds using artificially amended 20 L water matrices (deionized water with sterile dust, autoclaved wetland water, and wetland water). The optimal protoc

SUBMITTER: Hubbard LE 

PROVIDER: S-EPMC11680412 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature