Harnessing Nature-Inspired Catechol Amino Acid to Engineer Sticky Proteins and Bacteria.

ABSTRACT: 3,4-Dihydroxy-L-phenylalanine (DOPA) serves as a post-translational modification amino acid present in mussel foot proteins. Mussels exploit the exceptional adhesive properties of DOPA to adhere to a wide range of surfaces. This study presents the development of sticky proteins and bacteria through the site-specific incorporation of DOPA using Genetic Code Expansion Technology. Through the optimization of the DOPA incorporation system, proteins containing DOPA demonstrate significantly improved binding abilities to various organic and metallic materials. The material-binding capabilities of DOPA to combat different types of biofoulings are harnessed by integrating it into intrinsically disordered proteins. Beyond the creation of adhesive proteins for anti-biofouling purposes, this highly e

SUBMITTER: Zhang M 

PROVIDER: S-EPMC11680460 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature