Initial pig developmental stage influences intestinal organoid growth but not cellular composition.

ABSTRACT:

Background

Intestinal organoids are promising tools in the context of animal experiment reduction but a thorough characterization of the impact of the origin of intestinal stem cells (ISC) on organoid phenotype is needed to routinely use this cellular model. Our objective was to evaluate the effect of ISC donor age on the growth, morphology and cellular composition of intestinal organoids derived from pig.

Methods

Organoids were derived from jejunal and colonic ISC obtained from 1-, 7-, 28-, 36- and 180-day-old pigs and passaged three times.

Results

We first confirmed by qPCR that the expression of 18% of the >80 studied genes related to various intestinal functions differed between jejunal and colonic organoids after two passages (p < 0.05). Growth and morphology of

SUBMITTER: Duchesne C 

PROVIDER: S-EPMC11680479 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature