Carbapenem-induced β-lactamase-isoform expression trends in Acinetobacter baumannii.

ABSTRACT: Carbapenem-resistant Acinetobacter baumannii (CRAb) is an urgent bacterial threat to public health, with only a few treatment options and a > 50% fatality rate. Although several resistance mechanisms are understood, it is still impossible to predict which mutations are most likely to occur. Here, we demonstrate that independent samples of Ab, exposed to different carbapenems with escalating concentrations, show concentration- and carbapenem-dependent trends in β-lactamase-isoform expression. This result, based on the isoforms identified through label-free-quantification LC-MS/MS measurements of cell-free, gel-separated β-lactamases, suggests that the appearance of antibiotic resistance may be have some predictability based on structural factors. Additionally, the identified minor isoforms

SUBMITTER: Benin BM 

PROVIDER: S-EPMC11680855 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature