Genetic factors regulating Plasmodium falciparum gametocytogenesis identified by phenotypic screens.

ABSTRACT: Successful transmission of Plasmodium falciparum from one person to another relies on the complete intraerythrocytic development of non-pathogenic sexual gametocytes infectious for anopheline mosquitoes. Understanding the genetic factors that regulate gametocyte development is vital for identifying transmission-blocking targets in the malaria parasite life cycle. Toward this end, we conducted a forward genetic study to characterize the development of gametocytes from sexual commitment to mature stage V. We described a new analysis pipeline for the piggyBac transposon-based mutagenesis phenotypic screen to identify genes that influence both early and late gametocyte stages. We classified individual mutants that increased or decreased parasite abundance as the hypoproducer and hyperproducer

SUBMITTER: Pires CV 

PROVIDER: S-EPMC11680961 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature