Integrated taxonomy to advance species delimitation of the Anopheles maculipennis complex.

ABSTRACT: The Anopheles maculipennis complex consists of several mosquito species, including some primary malaria vectors. Therefore, the presence of a species in a particular area significantly affects public health. In this study, 1252 mosquitoes were collected in northern Italy, representing four identified species of the Anopheles maculipennis complex (Anopheles daciae sp. inq., Anopheles maculipennis s. s., Anopheles atroparvus and Anopheles melanoon). The sequences of two DNA markers, mitochondrial cytochrome c oxidase I (COI) and nuclear internal transcribed spacer 2 (ITS2), were generated. DNA-based species delimitation analyses were performed, incorporating public sequences, with distance-based and coalescent tree-based methods to confirm the actual species boundaries within the complex. Wh

SUBMITTER: Calzolari M 

PROVIDER: S-EPMC11681070 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature