Seed-specific expression of AtWRI1 enhanced the yield of cotton seed oil.

ABSTRACT: The WRINKLED1 (WRI1) transcription factor controls carbon flow in plants through regulating the expression of glycolysis and fatty acid biosynthesis genes. The role of Gossypium hirsutum WRINKLED1 (GhWRI1) in seed-oil accumulation still needs to be explored. Multiple sequence alignment of WRI1 proteins confirmed the presence of two conserved AP2 domains. The amino acid sequence of GhWRI1 exhibited 62% homology with AtWRI1 and phylogenetic analysis showed that GhWRI1 and AtWRI1 originated from common ancestors. Comparison of three dimensional structures of AtWRI1 and GhWRI1 indicated the presence of an altered alpha helix on the C-terminus that harbours spore coat protein domain in A. thaliana and other members of Brassicaceae but Kin17 domain in G.hirsutum. In the present study, we constru

SUBMITTER: Butt M 

PROVIDER: S-EPMC11681231 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature