MiRNA-6236 Regulation of Postischemic Skeletal Muscle Angiogenesis.

ABSTRACT:

Background

Peripheral arterial disease affects >200 million people worldwide and is characterized by impaired blood flow to the lower extremities. There are no effective medical treatments available. Using the mouse hind-limb ischemia model and miRNA sequencing, we identified a novel miRNA, miR-6236, whose expression significantly elevated in ischemic mouse limbs compared with nonischemic limbs. The role of miR-6236 in general or in postischemic angiogenesis is not known. Here we describe its role using in vivo and in vitro models of peripheral arterial disease.

Methods and results

In primary mouse and human endothelial cells, we studied the effect of simulated ischemia on miR-6236 expression and assessed its role in cell viability, apoptosis, migration, and tube formation d

SUBMITTER: Mani AM 

PROVIDER: S-EPMC11681604 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature