Deciphering a profiling based on multiple post-translational modifications functionally associated regulatory patterns and therapeutic opportunities in human hepatocellular carcinoma.

ABSTRACT:

Background

Posttranslational modifications (PTMs) play critical roles in hepatocellular carcinoma (HCC). However, the locations of PTM-modified sites across protein secondary structures and regulatory patterns in HCC remain largely uncharacterized.

Methods

Total proteome and nine PTMs (phosphorylation, acetylation, crotonylation, ubiquitination, lactylation, N-glycosylation, succinylation, malonylation, and β-hydroxybutyrylation) in tumor sections and paired normal adjacent tissues derived from 18 HCC patients were systematically profiled by 4D-Label free proteomics analysis combined with PTM-based peptide enrichment.

Results

We detected robust preferences in locations of intrinsically disordered protein regions (IDRs) with phosphorylated sites and other site biases t

SUBMITTER: Lao Y 

PROVIDER: S-EPMC11681642 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature