Distinct microbes, metabolites, and the host genome define the multi-omics profiles in right-sided and left-sided colon cancer.

ABSTRACT:

Background

Studies have reported clinical heterogeneity between right-sided colon cancer (RCC) and left-sided colon cancer (LCC). However, none of these studies used multi-omics analysis combining genetic regulation, microbiota, and metabolites to explain the site-specific difference.

Methods

Here, 494 participants from a 16S rRNA gene sequencing cohort (50 RCC, 114 LCC, and 100 healthy controls) and a multi-omics cohort (63 RCC, 79 LCC, and 88 healthy controls) were analyzed. 16S rRNA gene, metagenomic sequencing, and metabolomics analyses of fecal samples were evaluated to identify tumor location-related bacteria and metabolites. Whole-exome sequencing (WES) and transcriptome sequencing (RNA-seq) were conducted to obtain the mutation burden and genomic expression pattern.<

SUBMITTER: Liang L 

PROVIDER: S-EPMC11681701 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature