Biomarker potential of plasma cell-free DNA for cholangiocarcinoma.

ABSTRACT:

Background

To prevent the development of cholangiocarcinoma, an effective screening opisthorchiasis viverrini and/or differential diagnosis of and the cholangiocarcinoma is crucial needed. The level and quality of cfDNA in plasma are being investigated for their potential role as biomarkers in cholangiocarcinoma.

Methods

The study enrolled 43 healthy controls (N), 36 O. viverrini-infected subjects (OV), and 36 cholangiocarcinoma patients (CCA). Plasma cfDNA was quantified by fluorometry (Qubit 4), and qualified analysis, including % tumor fraction, ctDNA, ploidy number, and somatic copy number alteration (SCNA), was conducted using ULP-WGS and analyzed by iChorCNA. The statistical analysis and comparison among the groups were performed.

Results

The results show

SUBMITTER: Prasopdee S 

PROVIDER: S-EPMC11681853 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature