Hybridisation of in silico and in vitro bioassays for studying the activation of Nrf2 by natural compounds.

ABSTRACT: Oxidative stress, characterized by the damaging accumulation of free radicals, is associated with various diseases, including cardiovascular, neurodegenerative, and metabolic disorders. The transcription factor Nrf2 is pivotal in cellular defense against oxidative stress by regulating genes that detoxify free radicals, thus maintaining redox homeostasis and preventing cellular aging. Keap1 plays a regulatory role through its interaction with Nrf2, ensuring Nrf2 degradation under homeostatic conditions and facilitating its stabilization and nuclear translocation during oxidative stress. In the initial stage of our study, we conducted in vitro assays on HaCaT cells, a human keratinocyte cell line, to measure the expression levels of Nrf2 to reveal the activity of promising medicinal plants,

SUBMITTER: Nguyen BQH 

PROVIDER: S-EPMC11682235 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature