Flow-cytometry reveals mitochondrial DNA accumulation in <i>Saccharomyces cerevisiae</i> cells during cell cycle arrest.

ABSTRACT: Mitochondria are semi-autonomous organelles containing their own DNA (mtDNA), which is replicated independently of nuclear DNA (nDNA). While cell cycle arrest halts nDNA replication, mtDNA replication continues. In Saccharomyces cerevisiae, flow cytometry enables semi-quantitative estimation of mtDNA levels by measuring the difference in signals between cells lacking mtDNA and those containing mtDNA. In this study, we used flow cytometry to investigate mtDNA accumulation in yeast cells under G1 and G2 phase cell cycle arrest conditions utilising thermosensitive mutants cdc4-3 and cdc15-2. In line with the previous studies, cell cycle arrest induced a several-fold accumulation of mtDNA in both mutants. The total DNA levels in arrested cells correlated with cell forward

SUBMITTER: Potapenko EY 

PROVIDER: S-EPMC11683134 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature