Alpha-1 antitrypsin inhibits pertussis toxin.

ABSTRACT: Pertussis (whooping cough) is a vaccine-preventable but re-emerging, highly infectious respiratory disease caused by Bordetella pertussis. There are currently no effective treatments for pertussis, complicating care for nonvaccinated individuals, especially newborns. Disease manifestations are predominantly caused by pertussis toxin (PT), a pivotal virulence factor classified as an ADP-ribosylating AB-type protein toxin. In this work, an unbiased approach using peptide libraries, bioassay-guided fractionation and mass spectrometry revealed α₁-antitrypsin (α₁AT) as a potent PT inhibitor. Biochemistry-, cell culture-, and molecular modeling-based in vitro experimentation demonstrated that the α₁AT mode of action is based on blocking PT-binding to the host tar

SUBMITTER: Lietz S 

PROVIDER: S-EPMC11683240 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature