Tracing active members in microbial communities by BONCAT and click chemistry-based enrichment of newly synthesized proteins.

ABSTRACT: A comprehensive understanding of microbial community dynamics is fundamental to the advancement of environmental microbiology, human health, and biotechnology. Metaproteomics, defined as the analysis of all proteins present within a microbial community, provides insights into these complex systems. Microbial adaptation and activity depend to an important extent on newly synthesized proteins (nP), however, the distinction between nP and bulk proteins is challenging. The application of BONCAT with click chemistry has demonstrated efficacy in the enrichment of nP in pure cultures for proteomics. However, the transfer of this technique to microbial communities and metaproteomics has proven challenging and thus it has not not been used on microbial communities before. To address this, a new wor

SUBMITTER: Hellwig P 

PROVIDER: S-EPMC11683836 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature