OGT-mediated O-GlcNAcylation regulates macrophage polarization in heart failure via targeting IRF1.

ABSTRACT:

Background

Heart failure (HF) is a syndrome with complex etiology and high mortality in the world. Macrophage-related inflammation is involved in HF development. O-GlcNAcylation is a post-translational modification that affects pathological processes. This study aimed to investigate the role of O-GlcNAcylation in HF, especially its effect on macrophage polarization.

Methods

Raw264.7 cells were treated with lipopolysaccharide (LPS) to induce pro-inflammatory macrophages. HF mice were generated by transverse aortic constriction (TAC). After knockdown of OGT or overexpressing IRF1, macrophage polarization was evaluated using quantitative real-time polymerase chain reaction and flow cytometry. Underlying mechanism was analyzed using bioinformatic analysis, co-immunoprecipitation

SUBMITTER: Jing G 

PROVIDER: S-EPMC11684060 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature