Integrated Profiling of Extracellular Vesicle microRNA Impact on Trabecular Meshwork mRNA Expression: Insights From Microarray Analysis.

ABSTRACT:

Purpose

Extracellular vesicles (EVs) secreted by non-pigmented ciliary epithelial (NPCE) cells under oxidative stress may contribute to primary open-angle glaucoma (POAG) pathogenesis by altering gene expression in human trabecular meshwork (HTM) cells. This study investigated the impact of microRNAs (miRNAs) carried by NPCE-derived EVs on HTM cell gene expression under oxidative stress conditions.

Methods

NPCE cells were exposed to oxidative stress, and EVs were isolated from control and stressed cells. HTM cells were treated with these EVs, followed by microarray analysis to identify differentially expressed miRNAs in EVs and messenger RNAs (mRNAs) in HTM cells. Bioinformatics analysis was used to explore miRNA-mRNA interactions, enriched Gene Ontology (GO) terms, and miRN

SUBMITTER: Cohen-Davidi E 

PROVIDER: S-EPMC11684117 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature