Kv3-Expressing Cells Present More Elaborate N-Glycans with Changes in Cytoskeletal Proteins, Neurite Structure and Cell Migration.

ABSTRACT: The cues contained by N-glycans relay the quality, cellular destination, and interactions of proteins, thereby, impacting cellular physiology. Voltage-gated K⁺ (Kv3) channels have two conserved N-glycosylation sites which are vital for Kv3 channel activity, and primary motor neuron development. Our previous studies showed that the parental (NB_1) and N-glycan mutant (NB_1(-Mgat1), NB_1(-Mgat2), and NB_1(-Mgat3)) Neuroblastoma (NB) cell lines have compromised N-acetylglucosaminyltransferase activities: GnT-I, GnT-II, or GnT-III. Herein, we stably expressed Kv3.1b in the parental and engineered N-glycan mutant Neuroblastoma (NB) cell lines to examine how changes in N-glycans alter the cytoskeleton, and subsequently cellular properties. MALDI-TOF MS verified that the parental and m

SUBMITTER: Hall MK 

PROVIDER: S-EPMC11684427 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature