A High Resolution Melting Analysis (HRM) PCR assay for the detection and identification of Old World Leishmania species.

ABSTRACT:

Background

Cutaneous Leishmaniases (CL), highly endemic in Africa and Mediterranean region, are caused by different Leishmania parasite species. Accurate species identification is crucial for effective diagnosis, treatment, and control of these diseases, but traditionally relies on DNA-based methods. High Resolution Melting analysis PCR (HRM PCR) provides rapid results and precise differentiation based on nucleotide variations. We hypothesized that the Strumpellin gene of Leishmania could serve as an effective target for developing a HRM PCR method for the rapid and efficient detection and identification of Leishmania species in CL diagnosis.

Methodology

The Strumpellin gene was investigated in Trypanosomatidae family using bioinformatics and phylogenetic approaches to explo

SUBMITTER: Saadi-Ben Aoun Y 

PROVIDER: S-EPMC11684767 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature