TCR transgenic clone selection guided by immune receptor analysis and single-cell RNA expression of polyclonal responders.

ABSTRACT: Since the precursor frequency of naive T cells is extremely low, investigating the early steps of antigen-specific T cell activation is challenging. To overcome this detection problem, adoptive transfer of a cohort of T cells purified from T cell receptor (TCR) transgenic donors has been extensively used but is not readily available for emerging pathogens. Constructing TCR transgenic mice from T cell hybridomas is a labor-intensive and sometimes erratic process, since the best clones are selected based on antigen-induced CD69 upregulation or IL-2 production in vitro, and TCR chains are polymerase chain reaction (PCR)-cloned into expression vectors. Here, we exploited the rapid advances in single-cell sequencing and TCR repertoire analysis to select the best clones without hybridoma selecti

SUBMITTER: Debeuf N 

PROVIDER: S-EPMC11684785 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature