Investigating the prognostic utility of GSTP1 promoter methylation in prostate cancer.

ABSTRACT:

Objectives

We aim to determine the prognostic significance of glutathione S-transferase Pi 1 DNA methylation (mGSTP1) in two independent prostate cancer cohorts with long-term clinical follow-up data.

Subjects/patients and methods

We first re-examined a published, in-house whole genome bisulphite sequencing (WGBS) prostate cancer dataset, derived from radical prostatectomy (RP) tissue (n = 15) with median follow-up 19.5 years, to confirm and visualise the association between mGSTP1 and patient mortality. To validate prognostic significance, we used a quantitative methylation-specific head-loop (MS-HL) assay to measure mGSTP1 levels in a larger, independent cohort (n = 186), and performed univariable and multivariable Cox survival ana

SUBMITTER: Pidsley R 

PROVIDER: S-EPMC11685177 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature