Rapid detection of cardamom mosaic virus in crude plant extracts using reverse transcription-recombinase polymerase amplification-lateral flow assay (RT-RPA-LFA).

ABSTRACT: Cardamom mosaic virus causing mosaic/katte disease is the most destructive virus infecting cardamom. The development of effective diagnostic assays is essential for the production of virus-free plants, as the primary spread of the virus occurs through vegetative propagation. Currently used PCR-based assays are not suitable for Point-of-Care testing, require sophisticated equipment, and are time-consuming. Hence, in the present study, an assay based on reverse transcription-recombinase polymerase amplification (RT-RPA) combined with lateral flow assay (RT-RPA-LFA) was optimized for the specific, and sensitive detection of CdMV. The forward and reverse primers selected for RT-RPA were labeled with 6-carboxyfluorescein (FAM) and biotin respectively at the 5´end. The tedious total RNA p

SUBMITTER: Greeshma M 

PROVIDER: S-EPMC11685339 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature