Noncanonical calcium binding motif controls folding of HopQ1, a Pseudomonas syringae type III secretion effector, in a pH-dependent manner.

ABSTRACT: Virulence of many gram-negative bacteria relies upon delivery of type three effectors into host cells. To pass through the conduit of secretion machinery the effectors need to acquire an extended conformation, and in many bacterial species specific chaperones assist in this process. In plant pathogenic bacterium Pseudomonas syringae, secretion of only few effectors requires the function of chaperones. This raises a question how chaperone-independent effectors achieve an appropriate conformation for the secretion. One such mechanism was previously described for AvrPto. It contains a pH-sensitive switch, which is involved in unfolding of the effector at the mildly acidic pH corresponding to the pH value of the bacterial cytosol, and refolding at the neutral pH. Therefore, it was proposed tha

SUBMITTER: Giska F 

PROVIDER: S-EPMC11685791 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature