GRAPE-WEB: An automated computational redesign web server for improving protein thermostability.

ABSTRACT: We have developed the GReedy Accumulated strategy for Protein Engineering (GRAPE) to improve enzyme stability across various applications, combining advanced computational methods with a unique clustering and greedy accumulation approach to efficiently explore epistatic effects with minimal experimental effort. To make this strategy accessible to nonexperts, we introduced GRAPE-WEB, an automated, user-friendly web server that allows the design, inspection, and combination of stabilizing mutations without requiring extensive bioinformatics knowledge. GRAPE-WEB's robust performance and accessibility provide a comprehensive and adaptable approach to protein thermostability design, suitable for both newcomers and experienced practitioners in the field. The web server is accessible at https://g

SUBMITTER: Sun J 

PROVIDER: S-EPMC11685837 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature