Parallel single-cell metabolic analysis and extracellular vesicle profiling reveal vulnerabilities with prognostic significance in acute myeloid leukemia.

ABSTRACT: Acute myeloid leukemia (AML) is an aggressive disease with a high relapse rate. In this study, we map the metabolic profile of CD34⁺(CD38^low/-) AML cells and the extracellular vesicle signatures in circulation from AML patients at diagnosis. CD34⁺ AML cells display high antioxidant glutathione levels and enhanced mitochondrial functionality, both associated with poor clinical outcomes. Although CD34⁺ AML cells are highly dependent on glucose oxidation and glycolysis for energy, those from intermediate- and adverse-risk patients reveal increased mitochondrial dependence. Extracellular vesicles from AML are mainly enriched in stem cell markers and express antioxidant GPX3, with their profiles showing potential prognostic value. Extracellular vesicl

SUBMITTER: Forte D 

PROVIDER: S-EPMC11685939 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature