Real-time monitoring by interferometric light microscopy of phage suspensions for personalised phage therapy.

ABSTRACT: Phage therapy uses viruses (phages) against antibiotic resistance. Tailoring treatments to specific patient strains requires stocks of various highly concentrated purified phages. It, therefore, faces challenges: titration duration and specificity to a phage/bacteria couple; purification affecting stability; and highly concentrated suspensions tending to aggregate. To address these challenges, interferometric light microscopy (ILM), characterising particles (size, concentration, and visual homogeneity) within minutes, was applied herein to anti-Staphylococcus aureus myovirus phage suspensions. Particle concentration was linearly correlated with phage infectious titre (R² > 0.97, slope: 3 particles/plaque forming units (PFU)) at various degrees of purification, allowing to approx

SUBMITTER: Lapras B 

PROVIDER: S-EPMC11686143 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature