Cross-species stability of reference genes in medicinal plants Arnica montana and Arnica chamissonis.

ABSTRACT: A better understanding of secondary metabolites biosynthesis requires comprehensive research at the molecular level. Although the medicinal importance of secondary metabolites extracted from Arnica spp. has been well documented, the very plants themselves have been poorly studied. Characterization of biosynthetic pathways and their complex regulatory mechanisms may be enhanced via transcriptomic approach. To ensure the reliability of data, RT-qPCR based gene expression studies should be preceded by the reference genes selection step. Here, ten candidate reference genes were analyzed in terms of their expression stability in developing flowers of two arnica species, Arnica montana and Arnica chamissonis. After evaluating their expression stability with four distinct algorithms (geNorm, Norm

SUBMITTER: Sozoniuk M 

PROVIDER: S-EPMC11686183 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature