Genes involved in DMSO-mediated yield increase of entomopathogenic nematodes.

ABSTRACT: Entomopathogenic nematodes (EPNs) associated with their symbiotic bacteria can effectively kill insect pests, in agriculture, forestry and floriculture. Industrial-scale production techniques for EPNs have been established, including solid and liquid monoculture systems. It is found that supplement of 0.01% dimethyl sulfoxide (DMSO) to the culture medium significantly enhances the recovery and infective juvenile yield of these nematodes. However, the specific nematode genes responding to DMSO is not yet clear. This study identified differentially expressed genes and associated enriched pathways by analyzing the transcriptomic response of Heterorhabditis bacteriophora H06 in the liquid medium containing 0.01% DMSO, and validated the function of the selected genes using RNA interference (RNA

SUBMITTER: Liu X 

PROVIDER: S-EPMC11686212 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature