Novel approach to mapping of resistance mutations in whole genomes by using restriction enzyme modulation of transformation efficiency.

ABSTRACT: Restriction enzyme modulation of transformation efficiencies (REMOTE) is a method that makes use of genome restriction maps and experimentally observed differences in transformation efficiencies of genomic DNA restriction digests to discover the location of mutations in genomes. The frequency with which digested genomic DNA from a resistant strain transforms a susceptible strain to resistance is primarily determined by the size of the fragment containing the resistance mutation and the distance of the mutation to the end of the fragment. The positions of restriction enzyme cleavage sites immediately flanking the resistance mutation define these parameters. The mapping procedure involves a process of elimination in which digests that transform with high frequency indicate that the restricti

SUBMITTER: Lerner CG 

PROVIDER: S-EPMC1168657 | biostudies-literature | 2005 Jul

REPOSITORIES: biostudies-literature