Development of an optimized protocol for protoplast-to-plant regeneration of selected varieties of Brassica oleracea L.

ABSTRACT:

Background

Brassica oleracea L. is a key plant in the Brassicaceae family, known for popular vegetables like cabbage, broccoli, kale and collard. Collard (B. oleracea var. viridis) is a non-heading leafy vegetable grown in urban farms and community gardens in the United States and Europe. Improving collard and other Brassica germplasm can benefit from both traditional and new plant breeding technologies (NPBTs), such as CRISPR-Cas mediated transformation. An efficient transformation or protoplast fusion can only be achieved with a robust and reproducible protocol for protoplast-to-plant regeneration. This research focuses on optimizing in vitro culture conditions to enhance cell divisions, microcallus formation, and the regeneration of shoots and roots in four Brassica oleracea var

SUBMITTER: Stelmach-Wityk K 

PROVIDER: S-EPMC11686882 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature