Differential gene expression in benznidazole-resistant Trypanosoma cruzi parasites.

ABSTRACT: We analyzed the differential gene expression among representative Trypanosoma cruzi stocks in relation to benznidazole exposures using a random differentially expressed sequences (RADES) technique. Studies were carried out with drug pressure both at the natural susceptibility level of the wild-type parasite (50% inhibitory concentration for the wild type) and at different resistance levels. The pattern of differential gene expression performed with resistant stocks was compared to the population structure of this parasite, established by random amplified polymorphic DNA analysis and multilocus enzyme electrophoresis. A RADES band polymorphism was observed, and over- or underexpression was linked to the resistance level of the stock. The analysis of RADES bands suggested that different prod

SUBMITTER: Villarreal D 

PROVIDER: S-EPMC1168707 | biostudies-literature | 2005 Jul

REPOSITORIES: biostudies-literature