A chitosan/acellular matrix-based neural graft carrying mesenchymal stem cells to promote peripheral nerve repair.

ABSTRACT:

Background

Treatment of peripheral nerve defects is a major concern in regenerative medicine. This study therefore aimed to explore the efficacy of a neural graft constructed using adipose mesenchymal stem cells (ADSC), acellular microtissues (MTs), and chitosan in the treatment of peripheral nerve defects.

Methods

Stem cell therapy with acellular MTs provided a suitable microenvironment for axonal regeneration, and compensated for the lack of repair cells in the neural ducts of male 8-week-old Sprague Dawley rats.

Results

In vitro, acellular MTs retained the intrinsic extracellular matrix and improved the narrow microstructure of acellular nerves, thereby enhancing cell functionality. In vivo neuroelectrophysiological studies, gait analysis, and sciatic nerve histolo

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC11687163 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature