NST3 induces ectopic transdifferentiation, forming secondary walls with diverse patterns and composition in Arabidopsis thaliana.

ABSTRACT:

Background and aims

The master transcription factor NAC SECONDARY WALL THICKENING PROMOTING FACTOR3 (NST3), also known as SND1, plays a pivotal role in regulating secondary cell wall (SCW) development in interfascicular and xylary fibres in Arabidopsis thaliana. Despite progress in understanding SCW assembly in xylem vessel-like cells, the mechanisms behind its assembly across different cell types remain unclear. Overexpression of NST3 or its homologue NST1 leads to reduced fertility, posing challenges for studying their impact on secondary wall formation. This study aimed to develop a tightly regulated dexamethasone (DEX)-inducible expression system for NST3 and NST1 to elucidate the structure and assembly of diverse SCWs.

Methods

Using the DEX-inducible system, we characte

SUBMITTER: Tamadaddi C 

PROVIDER: S-EPMC11687626 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature