Whole-genome resequencing reveals collagen-related genes in Kele pigs.

ABSTRACT:

Objective

To verify the accuracy of collagen-specific SNP mutation loci of Kele pigs selected by whole genome resequencing, and to excavate collagen-related genes of Kele pigs, so as to lay a foundation for further molecular selection.

Methods

Based on whole genome resequencing, candidate genes related to collagen trait of Kele pig were screened for gene annotation. Through KEGG and GO enrichment analysis of differential genes, we selected four genes that may affect collagen trait of collagen pig, namely COL9A1, COL6A5, COL4A3 and COL4A4. Then 14 specific SNP sites were randomly selected from the four genes for sanger sequencing verification, and finally RT-qPCR was used to verify the expression levels of related genes in different tissues of Kele pigs.

Results

Our se

SUBMITTER: Zhang YD 

PROVIDER: S-EPMC11687657 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature