Preliminary exploration of metagenomic sequencing for pathogenic identification in infectious uveitis.

ABSTRACT:

Purpose

To evaluate the advantages and clinical utility of metagenomic sequencing (MGS) in diagnosing infectious uveitis pathogens.

Methods

A retrospective study was conducted on 20 infectious uveitis patients (20 eyes) who received treatments at the Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine from May 2020 to February 2021. Anterior aqueous humor of the patients was collected and analyzed using MGS. Then, pathogenic microorganisms that cause uveitis were identified through bioinformatic analyses based on the sequencing data of MGS. Finally, the pathogens identified by MGS were verified using both enzyme-linked immune sorbent assay (ELISA) and quantitative PCR (qPCR).

Results

MGS was used to detect viral pathogens in four patients, b

SUBMITTER: Yu J 

PROVIDER: S-EPMC11688260 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature