Rapid and reproducible generation of glioblastoma spheroids for high-throughput drug screening.

ABSTRACT: Identifying new substances that could potentially be used for tumor therapy and the precise analysis of their spectrum of action requires models that are as similar as possible to the tumor present in the patient. Traditionally, two-dimensional (2D) cell cultures are used. However, these only resemble solid tumors to a limited extent. More realistic in vivo models, such as tissue cultures, which are invaluable for the final analysis of the effect of new substances, are unsuitable for high-throughput screening (HTS), such as substance library screening. Therefore, we addressed which parameters need to be optimized to produce 3D cultures suitable for HTS using established tumor cell lines and ultra-low attachment plates, and we tested which experimental parameters need to be considere

SUBMITTER: Bach C 

PROVIDER: S-EPMC11688379 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature