A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice.

ABSTRACT: Co-occurrence of multiple mycotoxins is a growing global food safety concern due to their harmful effects on humans and animals. This study developed an eco-friendly sample preparation method and an innovative multiplex microarray-based lateral flow immunoassay, using a novel portable reader for on-site simultaneous determination of five regulated mycotoxins-aflatoxin B₁, T-2 toxin, zearalenone, deoxynivalenol, and fumonisin B₁ in rice. The eco-friendly and ultrafast extraction procedure utilizes a bio-based solvent. Principally, the microarray signals generated through a novel luminescent organic dye were captured to quantify mycotoxin levels in samples using a portable reader installed with a user-friendly interface. The assay demonstrates accurate detection and qua

SUBMITTER: Adunphatcharaphon S 

PROVIDER: S-EPMC11688421 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature