A new approach for the analysis of bacterial microarray-based Comparative Genomic Hybridization: insights from an empirical study.

ABSTRACT:

Background

Microarray-based Comparative Genomic Hybridization (M-CGH) has been used to characterize the extensive intraspecies genetic diversity found in bacteria at the whole-genome level. Although conventional microarray analytical procedures have proved adequate in handling M-CGH data, data interpretation using these methods is based on a continuous character model in which gene divergence and gene absence form a spectrum of decreasing gene conservation levels. However, whereas gene divergence may yet be accompanied by retention in gene function, gene absence invariably leads to loss of function. This distinction, if ignored, leads to a loss in the information to be gained from M-CGH data. We present here results from experiments in which two genome-sequenced strains of C. jejun

SUBMITTER: Taboada EN 

PROVIDER: S-EPMC1168892 | biostudies-literature | 2005 May

REPOSITORIES: biostudies-literature