Deletion and allelic exchange of the Aspergillus fumigatus veA locus via a novel recyclable marker module.

ABSTRACT: Detailed evaluation of gene functions in an asexual fungus requires advanced methods of molecular biology. For the generation of targeted gene deletions in the opportunistic pathogen Aspergillus fumigatus we designed a novel blaster module allowing dominant selection of transformants due to resistance to phleomycin as well as dominant (counter)selection of a Cre recombinase-mediated marker excision event. For validation purposes we have deleted the A. fumigatus pabaA gene in a wild-type isolate by making use of this cassette. The resulting pabaA::loxP strain served as the recipient for subsequent targeting of the velvet locus. Homologous reconstitution of the deleted gene was performed by an allele whose expression is driven in a nitrogen source-dependent manner, as validated by Northern a

SUBMITTER: Krappmann S 

PROVIDER: S-EPMC1168958 | biostudies-literature | 2005 Jul

REPOSITORIES: biostudies-literature