Survey of bovine enterovirus in biological and environmental samples by a highly sensitive real-time reverse transcription-PCR.

ABSTRACT: Animal enteroviruses shed in the feces of infected animals are likely environmental contaminants and thus can be used as indicators of animal fecal pollution. Previous work has demonstrated that bovine enterovirus (BEV) present in bovine feces contaminates waters adjacent to cattle herds and that BEV-like sequences are also present in shellfish and in deer feces from the same geographical area. However, little information is available about the prevalence, molecular epidemiology, and genomic sequence variation of BEV field isolates. Here we describe an optimized highly sensitive real-time reverse transcription-PCR method to detect BEV RNA in biological and environmental samples. A combination of the amplification procedure with a previously described filtration step with electropositive fi

SUBMITTER: Jimenez-Clavero MA 

PROVIDER: S-EPMC1168977 | biostudies-literature | 2005 Jul

REPOSITORIES: biostudies-literature