Loss of the Endothelial Glycocalyx Component EMCN Leads to Glomerular Impairment.

ABSTRACT:

Background

EMCN (endomucin), an endothelial-specific glycocalyx component, was found to be highly expressed by the endothelium of the renal glomerulus. We reported an anti-inflammatory role of EMCN and its involvement in the regulation of VEGF (vascular endothelial growth factor) activity through modulating VEGFR2 (VEGF receptor 2) endocytosis. The goal of this study is to investigate the phenotypic and functional effects of EMCN deficiency using the first global EMCN knockout mouse model.

Methods

Global EMCN knockout mice were generated by crossing EMCN-floxed mice with ROSA26-Cre mice. Flow cytometry was used to analyze infiltrating myeloid cells in the kidneys. The ultrastructure of the glomerular filtration barrier was examined by transmission electron microscopy, wherea

SUBMITTER: Hu Z 

PROVIDER: S-EPMC11692785 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature