Hyperglycemia causes differential change in macrophage population in the lacrimal gland, conjunctiva and cornea.

ABSTRACT:

Background

Due to its location, the ocular surface is exposed to environmental microbes. Innate immune cells including macrophages are first line defense against infections. In vitro exposure to high glucose as well as diabetes-associated hyperglycemia has been shown to affect innate immune cell function and population. The present study was designed to examine the effect of diabetes-associated hyperglycemia on the lacrimal gland, conjunctiva and cornea macrophage population, phenotypic changes and cytokines/chemokines.

Methods

Mouse model of Streptozotocin-induced diabetes was used to induce hyperglycemia. Immunostaining for CD11b and F4/80 was performed to stain macrophages in whole mount cornea, conjunctiva and 50 µm lacrimal gland sections. Flowcytometry was perfo

SUBMITTER: Alfuraih S 

PROVIDER: S-EPMC11693596 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature